SORD ELISA Kit (Pig)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
Gene Aliases
L-iditol 2-dehydrogenase.
Reactivity
Porcine, Sus scrofa
Target
Sorbitol dehydrogenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SORD ELISA Kit (Pig) (OKEH08077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SORD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SORD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SORD captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)
Sensitivity
0.78ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig Sorbitol dehydrogenase
NCBI Gene Symbol
SORD
Protein Name
Q29318
Gene Name URL
SORD
CAS Number
7732-18-5
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
