IDH1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Isocitrate dehydrogenase (NADP (+) ) 1
Gene Aliases
Cytosolic NADP-isocitrate dehydrogenase; IDH; IDP; isocitrate dehydrogenase (NADP (+) ) 1, cytosolic; isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase 1, soluble; NADP (+) -specific ICDH; oxalosuccinate decarboxylase.
Gene ID
24479
Accession Number
NP_113698
Reactivity
Rat, Rattus norvegicus
Target
Isocitrate dehydrogenase [NADP] cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Idh1 ELISA Kit (Rat) (OKEH07005) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Idh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Idh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Idh1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into matrix = 96%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Isocitrate dehydrogenase [NADP] cytoplasmic
NCBI Gene Symbol
Idh1
Protein Name
P41562
Gene Name URL
Idh1
Nucleotide Accession Number
NM_031510
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
