CDC42 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Cell division cycle 42
Gene Aliases
Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein) ; cell division cycle 42 homolog.
Gene ID
64465
Accession Number
NP_741991.3
Reactivity
Rat, Rattus norvegicus
Target
Cell division control protein 42 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cdc42 ELISA Kit (Rat) (OKEH06308) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdc42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdc42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdc42 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
7.92 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Cell division control protein 42 homolog
Protein Length
191
NCBI Gene Symbol
Cdc42
Protein Name
Q8CFN2
Gene Name URL
Cdc42
CAS Number
7732-18-5
Nucleotide Accession Number
NM_171994.4
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