GPC1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Glypican 1
Gene Aliases
Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12; HSPGM12.
Gene ID
58920
Accession Number
NP_110455.1
Reactivity
Rat, Rattus norvegicus
Target
Glypican-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gpc1 ELISA Kit (Rat) (OKEH05959) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gpc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpc1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 95%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Glypican-1
Protein Length
558
NCBI Gene Symbol
Gpc1
Protein Name
P35053
Gene Name URL
Gpc1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_030828.1
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