KLF13 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Kruppel-like factor 13
Gene Aliases
0610043C13Rik;9430029L20Rik; basic transcription element-binding protein 3; Bteb3; BTE-binding protein 3; C2-H2 zinc finger protein; erythroid transcription factor FKLF-2; FKLF-; FKLF2; FKLF-2; Klf1; Krueppel-like factor 13; N; NSLP1; RANTES factor of late activated T-lymphocytes 1; RFL; RFLA; RFLAT1; RFLAT-1; transcription factor BTEB3.
Gene ID
50794
Accession Number
NP_067341.2
Reactivity
Mouse, Mus musculus
Target
Krueppel-like factor 13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Klf13 ELISA Kit (Mouse) (OKEH05899) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Klf13 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Klf13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Klf13 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.9%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Krueppel-like factor 13
Protein Length
289
NCBI Gene Symbol
Klf13
Protein Name
Q9JJZ6
Gene Name URL
Klf13
CAS Number
7732-18-5
Nucleotide Accession Number
NM_021366.3
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