TRIL ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
TLR4 interactor with leucine-rich repeats
Gene Aliases
AI316813, mKIAA0644, 1200009O22Rik
Gene ID
66873
Accession Number
NP_080093.1
Reactivity
Mouse
Target
Component of the TLR4 signaling complex. Mediate the innate immune response to bacterial lipopolysaccharide (LPS) leading to cytokine secretion.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Tril ELISA Kit (Mouse) (OKEH05531) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tril has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tril is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tril captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 86.9%
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse TLR4 interactor with leucine rich repeats
Protein Length
809
NCBI Gene Symbol
TRIL
Protein Name
TLR4 interactor with leucine rich repeats
Gene Name URL
TRIL
CAS Number
7732-18-5
Nucleotide Accession Number
NM_025817.4
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