SLC25A20 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20
Gene Aliases
1110007P09Rik; C78826; CAC; Cact; Carnitine/acylcarnitine translocase; mCA; mCAC; mitochondrial carnitine/acylcarnitine carrier protein; Solute carrier family 25 member 20.
Gene ID
57279
Accession Number
NP_065266.1
Reactivity
Mouse, Mus musculus
Target
Mitochondrial carnitine/acylcarnitine carrier protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc25a20 ELISA Kit (Mouse) (OKEH05375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc25a20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc25a20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc25a20 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 98.6%
Sensitivity
0.162 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Mitochondrial carnitine/acylcarnitine carrier protein
Protein Length
301
NCBI Gene Symbol
Slc25a20
Protein Name
Q9Z2Z6
Gene Name URL
Slc25a20
CAS Number
7732-18-5
Nucleotide Accession Number
NM_020520.4
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