SERPINB3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serpin family B member 3
Gene Aliases
HsT1196; protein T4-A; SCC; SCCA1; SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin B3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; squamous cell carcinoma antigen 1; SSCA1; T4-A.
Gene ID
6317
Accession Number
NP_008850.1
Reactivity
Homo sapiens, Human
Target
Serpin B3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINB3 ELISA Kit (Human) (OKEH04839) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINB3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINB3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINB3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/mL
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Human Serpin B3
Protein Length
390
NCBI Gene Symbol
SERPINB3
Protein Name
P29508
Gene Name URL
SERPINB3
Nucleotide Accession Number
NM_006919.2
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
