Dpp4 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Dipeptidylpeptidase 4
Gene Aliases
Cd26; dipeptidyl peptidase 4; dipeptidyl peptidase IV; Dpp; DPP IV; Dpp-4; T-cell activation antigen CD26; THA; THAM; thymocyte-activating molecule.
Gene ID
13482
Accession Number
NP_001153015.1
Reactivity
Mouse, Mus musculus
Target
Dipeptidyl peptidase 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dpp4 ELISA Kit (Mouse) (OKEH04543) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dpp4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dpp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dpp4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
39.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
87 KDa
Specificity
Natural and recombinant Mouse Dipeptidyl peptidase 4
Protein Length
760
NCBI Gene Symbol
Dpp4
Protein Name
P28843
Gene Name URL
Dpp4
Nucleotide Accession Number
NM_001159543.1
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