PSIP1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
PC4 and SFRS1 interacting protein 1
Gene Aliases
AA408851; AU015605; dense fine speckles 70; Dfs70; Ledgfa; Ledgfb; lens epithelium-derived growth factor; P; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; Psip2.
Gene ID
101739
Accession Number
NP_001277456.1
Reactivity
Mouse, Mus musculus
Target
PC4 and SFRS1-interacting protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Psip1 ELISA Kit (Mouse) (OKEH04198) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Psip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psip1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.095 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Mouse PC4 and SFRS1-interacting protein
Protein Length
528
NCBI Gene Symbol
Psip1
Protein Name
Q99JF8
Gene Name URL
Psip1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001290527.1
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