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LYZ ELISA Kit (Chicken)

Product Specifications

CAS Number

7732-18-5

Gene Name

Lysozyme (renal amyloidosis)

Gene Aliases

1,4-beta-N-acetylmuramidase C;4-beta-N-acetylmuramidase C; allergen Gal d IV; dystrophin; egg white lysozyme; lysozyme C; LYZC.

Gene ID

396218

Accession Number

NP_990612.1

Reactivity

Chicken, Gallus gallus

Target

Lysozyme C

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LYZ ELISA Kit (Chicken) (OKEH03977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LYZ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LYZ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LYZ captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

3.12-200ng/mL

Sensitivity

1.39 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

147 kDa

Specificity

Natural and recombinant Chicken Lysozyme C

Protein Length

147

NCBI Gene Symbol

LYZ

Protein Name

P00698

Gene Name URL

LYZ

Nucleotide Accession Number

NM_205281.1

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