CNP ELISA Kit (Rat)
Product Specifications
Gene Name
2',3'-cyclic nucleotide 3' phosphodiesterase
Gene Aliases
2',3'-cyclic-nucleotide 3'-phosphodiesterase; Cnp1; CNPase; CNPF; CNPI; CNPII.
Gene ID
25275
Accession Number
NP_036941.1
Reactivity
Rat, Rattus norvegicus
Target
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CNP ELISA Kit (Rat) (OKEH02454) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat CNP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CNP and incubated. The CNP found in the sample or standards competes with the biotinylated CNP for limited binding sites on the immobilized anti-Rat CNP antibody. Excess unbound biotinylated CNP and sample or standard CNP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CNP captured in the well and inversely proportional to the amount of CNP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Sensitivity
0.095 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 kDa
Specificity
Natural and recombinant Rat 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Protein Length
420
NCBI Gene Symbol
Cnp
Protein Name
P13233
Gene Name URL
Cnp
CAS Number
7732-18-5
Nucleotide Accession Number
NM_012809.2
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