LTB4R ELISA Kit (Human)
Product Specifications
Gene Name
Leukotriene B4 receptor
Gene Aliases
BLT1; BLTR; chemoattractant receptor-like 1; chemokine receptor-like 1; CMKRL1; G protein-coupled receptor 16; GPR16; G-protein coupled receptor 16; leukotriene B4 receptor 1; LTB4-R 1; LTB4R1; LTB4-R1; LTBR1; P2RY7; P2Y purinoceptor 7; P2Y7; purinergic receptor P2Y, G-protein coupled, 7.
Gene ID
1241
Accession Number
NP_001137391.1
Reactivity
Homo sapiens, Human
Target
Leukotriene B4 receptor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LTB4R ELISA Kit (Human) (OKEH02050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LTB4R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LTB4R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LTB4R captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.43 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Human Leukotriene B4 receptor 1
Protein Length
352
NCBI Gene Symbol
LTB4R
Protein Name
Q15722
Gene Name URL
LTB4R
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001143919.2
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