H3F3A ELISA Kit (Human)
Product Specifications
Gene Name
H3.3 histone A, H3.3 histone B
Gene Aliases
H3 histone family member 3A; H3 histone family member 3B; H3 histone, family 3A; H3 histone, family 3B (H3.3B) ; H3.3A; H3.3B; H3-3A; H3-3B; H3F3; H3F3A; H3F3B; histone H3.3.
Gene ID
3020, 3021
Accession Number
NP_001013721.2
Reactivity
Homo sapiens, Human
Target
Histone H3.3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology H3F3A ELISA Kit (Human) (OKEH01773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H3F3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H3F3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H3F3A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.181 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
15 kDa
Specificity
Natural and recombinant Human Histone H3.3
Protein Length
135
NCBI Gene Symbol
H3-3A, H3-3B
Protein Name
P84243
Gene Name URL
H3-3A, H3-3B
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001013699.2
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