GPX2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glutathione peroxidase 2
Gene Aliases
Gastrointestinal glutathione peroxidase; GI-GPx; glutathione peroxidase 2; glutathione peroxidase 2 (gastrointestinal) ; Glutathione peroxidase-gastrointestinal; glutathione peroxidase-related protein 2; GPRP; GPRP-2; GPx-2; GPx-GI; GSHPx-2; GSHPX-GI; selenoprotein GPX2.
Gene ID
2877
Accession Number
NP_002074.2
Reactivity
Homo sapiens, Human
Target
Glutathione peroxidase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GPX2 ELISA Kit (Human) (OKEH01009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPX2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Glutathione peroxidase 2
Protein Length
190
NCBI Gene Symbol
GPX2
Protein Name
P18283
Gene Name URL
GPX2
Nucleotide Accession Number
NM_002083.3
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
abx304197-01 20 µg
Interferon Induced Protein With Tetratricopeptide Repeats 5 (IFIT5) Antibody (HRP)
Ask
View Details 
abx304197-02 50 µg
Interferon Induced Protein With Tetratricopeptide Repeats 5 (IFIT5) Antibody (HRP)
Ask
View Details abx304197-03 100 µg
Interferon Induced Protein With Tetratricopeptide Repeats 5 (IFIT5) Antibody (HRP)
Ask
View Details