ERBB2 ELISA Kit (Rat)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog) ; C-erbB-2; epidermal growth factor receptor-related protein; HER2/neu; neu; NEU proto-oncogene; p185erbB2; p185neu; proto-oncogene c-ErbB-2; proto-oncogene NEU; receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2-like; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Tyrosine Protein Kinase erbB-2 (ErbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 96-105 101 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 88-101 95 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 96-105 101 serum (n=5) 96-105 101 EDTA plasma (n=5) 91-99 96 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 88-101 95 heparin plasma (n=5) 88-101 95

Component Amount Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Erbb2 Lyophilized Standard 2 x 40 ng 100X Biotinylated Erbb2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Erbb2 Lyophilized Standard 2 x 40 ng Erbb2 Lyophilized Standard 2 x 40 ng 100X Biotinylated Erbb2 Detector Antibody 120 uL 100X Biotinylated Erbb2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor Receptor 2 (EGFR2) . No significant cross-reactivity or interference between Epidermal Growth Factor Receptor 2 (EGFR2) and analogues was observed.