ETS1 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

AI196000; AI448617; avian erythroblastosis virus E26 oncogene 1; D230050P06; Ets-; Ets-1; p42Ets; p51Ets; p54; protein C-ets-1; Tp; Tpl1; tumor progression locus 1; vs.

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (By similarity) .

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery Range (%) Average (%) serum (n=5) 84-92 89 EDTA plasma (n=5) 95-102 99 heparin plasma (n=5) 85-101 97 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 84-92 89 serum (n=5) 84-92 89 EDTA plasma (n=5) 95-102 99 EDTA plasma (n=5) 95-102 99 heparin plasma (n=5) 85-101 97 heparin plasma (n=5) 85-101 97

Component Amount Anti-Ets1 Microplate 96 Wells (12 x 8 Well Strips) Ets1 Lyophilized Standard 2 x 10 ng 100X Biotinylated Ets1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ets1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Ets1 Microplate 96 Wells (12 x 8 Well Strips) Ets1 Lyophilized Standard 2 x 10 ng Ets1 Lyophilized Standard 2 x 10 ng 100X Biotinylated Ets1 Detector Antibody 120 uL 100X Biotinylated Ets1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

This assay has high sensitivity and excellent specificity for detection of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) . No significant cross-reactivity or interference between V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) and analogues was observed.