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CHUK Antibody (Phospho-Ser176/177)

Product Specifications

CAS Number

9007-83-4

Gene Name

Conserved helix-loop-helix ubiquitous kinase

Gene Aliases

IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha

Gene ID

1147; 3551; 16150; 84351

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human IKK Alpha/Beta around the phosphorylation site of S176/177. Location: 120-200aa.

Target

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

80 kDa

Fragment

IgG

Specificity

Phospho-IKK Alpha/Beta (S176/177) Polyclonal Antibody detects endogenous levels of IKK Alpha/Beta protein only when phosphorylated at S176/177.

NCBI Gene Symbol

CHUK, IKBKB

Host or Source

Rabbit

Gene Name URL

CHUKIKBKB

Curated Selection

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