PFKL antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

6-phosphofructokinase, liver type

Gene Aliases

PFK-B, PFK-L, ATP-PFK

Gene ID

5211

Accession Number

NP_002617.3

Reactivity

Human, Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

Target

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L) . PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

ICC, IF, IHC-FFPE, WB

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Varies by lot. See vial for concentration.

Format

Liquid// 0.1M Tris-Glycine (pH 7) with 10% glycerol and 0.01% Thimerosal

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

Reconstitution

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze/thaw cycles.