MKI67 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Antigen identified by monoclonal antibody Ki 67

Gene Aliases

Antigen identified by monoclonal antibody Ki-67 homolog; antigen Ki67 homolog; antigen KI-67 homolog; D630048A14Rik; Ki6; Ki-6; Ki67; Ki-67; proliferation marker protein Ki-67.

Gene ID

17345

Accession Number

NP_001074586.2

Reactivity

Mouse

Immunogen

Recombinant Ki-67 (Cys2386~Val2674) expressed in E.coli.

Target

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226) . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226) . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226) . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity) . Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251) . May play a role in chromatin organization (PubMed:26949251) . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Molecular Weight

325 kDa

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against Ki67P. It has been selected for its ability to recognize Ki67P in immunohistochemical staining and western blotting.