GLI3 Antibody
Product Specifications
Gene Name
GLI-Kruppel family member GLI3
Gene Aliases
Add; AI854843; anterior digit pattern deformity; AU023367; Bp; Bph; brachyphalangy; extra toes; GLI3 form of 190 kDa; GLI3 full length protein; GLI3 full-length protein; GLI3-190; GLI3FL; Pdn; polydactyly Nagoya; transcriptional activator GLI3; Xt; zinc finger protein GLI3.
Gene ID
14634
Accession Number
NP_032156.2
Reactivity
Mouse
Immunogen
Range: Ser303 - Glu561
Target
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
172 kDa
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against GLI3. It has been selected for its ability to recognize GLI3 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
Gli3
Host or Source
Rabbit
Protein Name
Transcriptional activator GLI3
Gene Name URL
Gli3
CAS Number
9007-83-4
Nucleotide Accession Number
NM_008130
Curated Selection
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