APOOL Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Apolipoprotein O like
Gene Aliases
AAIR8193; Apolipoprotein O-like; CXorf33; FAM121A; family with sequence similarity 121A; Mic27; MICOS complex subunit MIC27; MICOS27; mitochondrial contact site and cristae organizing system subunit 27; Protein FAM121A; UNQ8193.
Gene ID
139322
Swiss Prot
Q6UXV4
Accession Number
NP_940852.3
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human APOOL.
Target
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reconstitution
-20°C
Molecular Weight
29 kDa
Notes
Formerly GWB-ASE361
Fragment
IgG
Specificity
APOOL antibody detects endogenous levels of total APOOL protein.
NCBI Gene Symbol
APOOL
Host or Source
Rabbit
Protein Name
MICOS complex subunit MIC27
Gene Name URL
APOOL
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