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CYP3A4 Antibody

Product Specifications

Gene Name

Cytochrome P450 family 3 subfamily A member 4|cytochrome P450 family 3 subfamily A member 5

Gene Aliases

1,4-cineole 2-exo-monooxygenase;1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; albendazole monooxygenase (sulfoxide-forming) ; albendazole sulfoxidase; aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase; CP33; CP34; CP35; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; CYPIIIA5; cytochrome P450 3A3; cytochrome P450 3A4; cytochrome P450 3A5; cytochrome P450 HLp; cytochrome P450 HLp2; cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; cytochrome P450-PCN1; cytochrome P450-PCN3; flavoprotein-linked monooxygenase; glucocorticoid-inducible P450; HLP; microsomal monooxygenase; NF-25; nifedipine oxidase; P450C3; P450-III, steroid inducible; P450PCN1; P450PCN3; PCN3; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; VDDR3; xenobiotic monooxygenase.

Gene ID

1576|1577

Swiss Prot

P08684|P20815

Accession Number

NP_000768.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A4/5.

Target

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.|Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812) . Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

-20°C

Molecular Weight

57 kDa

Notes

Formerly GWB-ASE135

Specificity

Cytochrome P450 3A4/5 antibody detects endogenous levels of total Cytochrome P450 3A4/5 protein.

NCBI Gene Symbol

CYP3A4|CYP3A5

Host or Source

Rabbit

Protein Name

Cytochrome P450 3A4|Cytochrome P450 3A5

Gene Name URL

CYP3A4|CYP3A5

CAS Number

9007-83-4

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