EEF2K Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Eukaryotic elongation factor 2 kinase

Gene Aliases

Alternative protein EEF2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; HSU93850.

Gene ID

29904

Swiss Prot

O00418

Accession Number

NP_037434.1

Reactivity

Human|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human eEF2K.

Target

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid// PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+

Reconstitution

-20°C

Molecular Weight

82 kDa

Notes

Formerly GWB-ASC091

Fragment

IgG

Specificity

EEF2K (Ab-366) antibody detects endogenous levels of total eEF2K protein.

NCBI Gene Symbol

EEF2K

Host or Source

Rabbit

Protein Name

Eukaryotic elongation factor 2 kinase

Gene Name URL

EEF2K

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