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TCF3 Antibody (Phospho-Thr355)

Product Specifications

CAS Number

9007-83-4

Gene Name

Transcription factor 3

Gene Aliases

AGM8; bHLHb21; class B basic helix-loop-helix protein 21; E2A; E2A-HLF fusion transcript protein; E47; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; ITF1; kappa-E2-binding factor; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; p75; TCF-3; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) ; transcription factor 3 variant 3; transcription factor E2-alpha; transcription factor ITF-1; VDIR; VDR interacting repressor; vitamin D receptor-interacting repressor.

Gene ID

6929

Swiss Prot

P15923

Accession Number

NP_001129611.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human E2A around the phosphorylation site of Thr355.

Target

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

67 kDa

Notes

Formerly GWB-ASB983

Specificity

E2A (Phospho-Thr355) antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.

NCBI Gene Symbol

TCF3

Host or Source

Rabbit

Protein Name

Transcription factor E2-alpha

Gene Name URL

TCF3

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