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PPP1R1B Antibody (Phospho-Thr34)

Product Specifications

CAS Number

9007-83-4

Gene Name

Protein phosphatase 1 regulatory inhibitor subunit 1B

Gene Aliases

DARPP32; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; protein phosphatase 1 regulatory subunit 1B.

Gene ID

84152

Swiss Prot

Q9UD71

Accession Number

NP_001229393.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.

Target

Inhibitor of protein-phosphatase 1.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

22 kda

Notes

Formerly GWB-ASB644

Specificity

DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.

NCBI Gene Symbol

PPP1R1B

Host or Source

Rabbit

Protein Name

Protein phosphatase 1 regulatory subunit 1B

Gene Name URL

PPP1R1B

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