EEF2 Antibody (Phospho-Thr56)

Product Specifications

CAS Number

9007-83-4

Gene Name

Eukaryotic translation elongation factor 2

Gene Aliases

EEF-2; EF2; EF-2; elongation factor 2; epididymis secretory sperm binding protein; polypeptidyl-tRNA translocase; SCA26.

Gene ID

1938

Swiss Prot

P13639

Accession Number

NP_001952.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56.

Target

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Reconstitution

-20°C

Molecular Weight

95 kDa

Notes

Formerly GWB-ASB057

Fragment

IgG

Specificity

EEF2 (Phospho-Thr56) antibody detects endogenous levels of eEF2 only when phosphorylated at threonine 56.

NCBI Gene Symbol

EEF2

Host or Source

Rabbit

Protein Name

Elongation factor 2

Gene Name URL

EEF2

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