URM1 Antibody - N-terminal region
Product Specifications
Gene Aliases
C9orf74
Gene ID
81605
Swiss Prot
Q9BTM9
Accession Number
NP_112176
Reactivity
Human
Immunogen
The immunogen is a synthetic peptide directed towards the N-terminal region of Human URM1
Target
URM1 acts as a sulfur carrier required for 2-thiolation of mcm (5) S (2) U at tRNA wobble positions of cytosolic tRNA (Lys), tRNA (Glu) and tRNA (Gln) . URM1 serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm (5) S (2) U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
Partner Proteins
SEC23IP; DSTN; PTGES3; TXNL1; USP5; XPO1; VCL; PRDX2; PRDX1; ATP6V1A; CTU1; UBC; ASCC2; TBL3; CDK11B; UBA4
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
TLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWE
Applications
WB
Purification
Affinity purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
11kDa
Protein Length
101
NCBI Gene Symbol
URM1
Host or Source
Rabbit
Gene Name URL
URM1
CAS Number
9007-83-4
Curated Selection
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