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Recombinant Symbiobacterium thermophilum DNA mismatch repair protein MutS (mutS), partial

Product Specifications

Product Name Alternative

MutS; STH1757; DNA mismatch repair protein MutS

Abbreviation

Recombinant Symbiobacterium thermophilum mutS protein, partial

Gene Name

MutS

UniProt

Q67NK1

Expression Region

574-781aa

Organism

Symbiobacterium thermophilum (strain T / IAM 14863)

Target Sequence

YGYCRPLVDGSTVLELKGSRHPVLERVMEEGAFVPNDLLVDTGENRVLLITGPNMGGKSTVMRQAALAVILAQAGSFVPAESAHIGLVDRVFTRVGASDDLATGRSTFMVEMTEVANILHSATERSLVVLDEVGRGTATFDGLSIAWAITEHIHQAIGCRTLFATHYHELCELEGILPGVKNYSVAVMEKGEDIIFLRKLVRGGADRS

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

Molecular Weight

29.4 kDa

References & Citations

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.Nature 392:353-358 (1998)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

Curated Selection

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