Recombinant Human Islet amyloid polypeptide (IAPP), partial

Product Specifications

Product Name Alternative

PYY-I

Abbreviation

Recombinant Human IAPP protein, partial

Gene Name

IAPP

UniProt

P10997

Expression Region

34-70aa

Organism

Homo sapiens (Human)

Target Sequence

KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

Yeast

Field of Research

Signal Transduction

Relevance

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

Molecular Weight

5.9 kDa

References & Citations

The PP-fold solution structure of human polypeptide YY and human PYY3-36 as determined by NMR.Nygaard R., Nielbo S., Schwartz T.W., Poulsen F.M.Biochemistry 45:8350-8357 (2006)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.